A: Thin-Layer Chromatographic Identification Test 201—

B: The retention times of the peaks for curcumin, desmethoxycurcumin, and bisdesmethoxycurcumin in the chromatogram of the Test solution correspond to those in the chromatogram of the Standard solution for the appropriate USP Reference Standard, as obtained in the test for Content of curcuminoids.

Medium: water containing 1% sodium lauryl sulfate; 900 mL.

Apparatus 2: 100 rpm.

Time: 60 minutes.

Test solution— Combine 25-mL portions of the solution under test from each of the six dissolution vessels, and mix. Transfer 5 mL of the supernatant, accurately measured, to a 25-mL volumetric flask, dilute with Mobile phase to volume, and mix.

Tolerances— Not less than 75% of the content of curcumin is dissolved in 60 minutes.

Mobile phase, Standard solution 1, Standard solution 2, Standard solution 3, Standard solution 4, and Chromatographic system— Proceed as directed in the test for Content of curcuminoids under Turmeric.

Test solution— Weigh and finely powder the contents of not fewer than 20 Capsules. Transfer an accurately weighed quantity of the powder, equivalent to about 20 mg of curcuminoids, to a 50-mL volumetric flask, add about 30 mL of acetone, sonicate for 30 minutes, dilute with acetone to volume, mix, and centrifuge. Transfer 5 mL of the supernatant to a 50-mL volumetric flask, dilute with Mobile phase to volume, mix, and before injection pass through a filter having a 0.45-µm porosity.

Procedure— Proceed as directed in the test for Content of curcuminoids under Turmeric. Separately calculate the amount, in mg, of curcumin, desmethoxycurcumin, and bisdesmethoxycurcumin in the portion of the Capsules taken by the formula: in which C, rU, and rS are as defined therein. Calculate the content of curcuminoids, in mg, in the portion of Capsules taken by adding the amounts of curcumin, desmethoxycurcumin, and bisdesmethoxycurcumin.USP32